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prime script rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script rt reagent kit
    Prime Script Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pmc13101298-80-6-11
    Average 99 stars, based on 88195 article reviews
    prime script rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    cDNA Synthesis:

    Article Title: Resveratrol supplementation mitigates age-related declines in egg production, quality, and ovarian health of laying hens
    Article Snippet: .. Complementary DNA (cDNA) synthesis was performed with the PrimeScript RT Reagent Kit (RR047A, TaKaRa Bio Inc., Dalian, Liaoning, China) with genomic DNA removal performed. .. Quantitative real-time PCR amplification was conducted on an ABI Prism 7000 system (Applied Biosystems, Foster City, CA, USA) using SYBR Premix Ex Taq II (Takara Bio Inc., Dalian, Liaoning, China).

    Article Title: Dual targeting of NCF1 and NLRP3 by roburic acid orchestrates redox homeostasis and inhibits macrophage death in septic lung injury
    Article Snippet: .. Genomic DNA was removed and cDNA synthesis was performed using the PrimeScript RT Reagent Kit (TaKaRa RR037A, Japan) in accordance with the manufacturer's instructions, which included a dedicated gDNA Eraser step. ..

    Reverse Transcription:

    Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds
    Article Snippet: Total RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer's instructions. .. Then, 1 μg of total RNA was reverse-transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara). .. Quantitative PCR was performed in triplicate for each sample on an ABI Prism 7900 HT system (Applied Biosystems, USA) using SYBR Green reagent (Takara), with 10 ng of cDNA as the template.

    Article Title: Early nasal and lung transcriptomic profiles reveal pathways associated with divergent clinical outcomes following H7N1 high pathogenicity avian influenza virus infection
    Article Snippet: .. The total RNA was reverse-transcribed to cDNA using the PrimeScript RT reagent kit (Takara Bio, Inc.) according to the manufacturer’s instructions. .. Briefly, cDNA samples were preamplified using a Preamp Master Mix (Fluidigm Corporation) for 16 cycles.

    Article Title: Transcriptomic and hormonic analyses reveal the effects of xenia on anthocyanin biosynthesis in blueberry
    Article Snippet: Total RNA was extracted from fruit using an RNAprep pure Plant Kit (Tiangen, Beijing, China) following the manufacturer's instructions. .. Reverse transcription was performed using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan). .. PCR amplification was conducted according to the SYBR Premix Ex Taq II Kit protocol (Takara Bio, Shiga, Japan).

    Article Title: A dual-functional engineered exosome-laden hydrogel redirects endogenous neural stem cell fate for spinal cord injury repair
    Article Snippet: Finally the membrane was exposed for imaging using an enhanced chemiluminescence system (Bio-Rad, Hercules, CA, USA). .. Total RNA was extracted using a Total RNA Extraction Kit (R1200, Solarbio, Beijing, China). mRNA was reverse-transcribed into cDNA using the Primescript RT reagent Kit (RR037A, Takara, Tokyo, Japan). .. Subsequently, quantitative real-time PCR was performed using the LightCycler 96 system and TB Green Preix Ex Taq II (RR820B, Takara).

    Article Title: From N-of-1 to versatility in propionic acidemia: Antisense oligonucleotide-mediated skipping of a constitutive PCCA pseudoexon
    Article Snippet: The RNA concentrations were measured using a NanoDrop One spectrophotometer (Thermo Fisher Scientific). .. Equal amounts of total RNA extracted from fibroblasts were reverse transcribed into cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa Bio, Shiga, Japan) following the manufacturer’s protocol. ..

    RNA Extraction:

    Article Title: A dual-functional engineered exosome-laden hydrogel redirects endogenous neural stem cell fate for spinal cord injury repair
    Article Snippet: Finally the membrane was exposed for imaging using an enhanced chemiluminescence system (Bio-Rad, Hercules, CA, USA). .. Total RNA was extracted using a Total RNA Extraction Kit (R1200, Solarbio, Beijing, China). mRNA was reverse-transcribed into cDNA using the Primescript RT reagent Kit (RR037A, Takara, Tokyo, Japan). .. Subsequently, quantitative real-time PCR was performed using the LightCycler 96 system and TB Green Preix Ex Taq II (RR820B, Takara).



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