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primescript rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript rt reagent kit
    Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pm42897656-87-25-30
    Average 99 stars, based on 89544 article reviews
    primescript rt reagent kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Purification:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Ethanol Precipitation:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Reverse Transcription:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: .. Total RNA was extracted using the RNA Extraction Kit (Novizan, RC112- 01) and reverse- transcribed into cDNA with the PrimeScript RT Reagent Kit (TaKaRa, RR037A). .. qPCR was performed using iTaq Universal SYBR Green Supermix (Bio- Rad, 1 725 121) on a CFX Touch Real- Time PCR System (Bio- Rad).

    Article Title: ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns.
    Article Snippet: .. 2.6 Quantitative real-time PCR (qRT-PCR) Total RNA was isolated using TRIzol Invitrogen (#15596018, Invitrogen) according to the manufacturer’s instruction and was reverse transcribed by using PrimeScript RT reagent Kit (#RR037Q, Takara, Kusatsu, Shiga, Japan). .. Real time PCR was performed using TB Green Premix Ex Taq II (#RR820Q, Takara) on a Real time PCR system (#ABI7900HT, Applied Biosystems, Carlsbad, California).

    Article Title: Genistein targets Wnt5a to alleviate temporomandibular joint osteoarthritis by suppressing JNK/JUN-dependent MMP9 activation.
    Article Snippet: Temporomandibular joint osteoarthritis (TMJ-OA) is a progressive degenerative joint disease characterized by cartilage destruction, extracellular matrix (ECM) degradation, and chronic inflammation, yet effective disease-modifying therapeutic targets and pharmacological interventions remain limited.. Using a monosodium iodoacetate (MIA)-induced rat TMJ-OA model and interleukin-1β (IL-1β)-stimulated chondrocytes, we integrated transcriptomic analysis, chromatin accessibility profiling, and molecular screening to elucidate disease mechanisms and identify candidate compounds.. Integrated RNA sequencing (RNA-seq) analysis revealed Wnt family member 5a (Wnt5a) as a critical upstream regulator significantly associated with TMJ-OA progression, while assay for transposase-accessible chromatin using sequencing (ATAC-seq) uncovered an inflammatory chromatin landscape characterized by enhanced activator protein-1 (AP-1) activity and JUN-dependent transcriptional regulation of matrix metalloproteinase 9 (Mmp9).

    Article Title: Systematic engineering of COPII machinery and cell wall remodeling enables gram-level secretion of recombinant human serum albumin with native-like structural features in Kluyveromyces marxianus.
    Article Snippet: Recombinant human serum albumin (rHSA) is an important alternative to plasmaderived HSA, but its microbial production is constrained by the secretion burden associated with its large size and 17 intramolecular disulfide bonds.. Here, we developed a systematic secretory-network engineering strategy in the food-safe yeast Kluyveromyces marxianus.. The α-factor leader enabled efficient secretion and correct N-terminal processing of rHSA.

    cDNA Synthesis:

    Article Title: Genome-wide identification of the TIFY gene family in Sorghum bicolor and functional characterization of SbTIFY9 in salt stress tolerance.
    Article Snippet: The TIFY gene family is a plant-specific group of transcriptional regulators characterized by a conserved TIFY domain (TIF[F/Y]XG), participating in plant development, phytohormone signaling and abiotic stress responses.. In this study, 21 SbTIFY genes were genome-widely identified in sorghum (Sorghum bicolor L.).. According to phylogeny and protein structures, SbTIFY proteins were grouped into JAZ and ZML subfamilies, and the JAZ subfamily was further divided into five clades.

    RNA Extraction:

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: .. Total RNA was extracted using the RNA Extraction Kit (Novizan, RC112- 01) and reverse- transcribed into cDNA with the PrimeScript RT Reagent Kit (TaKaRa, RR037A). .. qPCR was performed using iTaq Universal SYBR Green Supermix (Bio- Rad, 1 725 121) on a CFX Touch Real- Time PCR System (Bio- Rad).

    Real-time Polymerase Chain Reaction:

    Article Title: ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns.
    Article Snippet: .. 2.6 Quantitative real-time PCR (qRT-PCR) Total RNA was isolated using TRIzol Invitrogen (#15596018, Invitrogen) according to the manufacturer’s instruction and was reverse transcribed by using PrimeScript RT reagent Kit (#RR037Q, Takara, Kusatsu, Shiga, Japan). .. Real time PCR was performed using TB Green Premix Ex Taq II (#RR820Q, Takara) on a Real time PCR system (#ABI7900HT, Applied Biosystems, Carlsbad, California).

    Article Title: Systematic engineering of COPII machinery and cell wall remodeling enables gram-level secretion of recombinant human serum albumin with native-like structural features in Kluyveromyces marxianus.
    Article Snippet: Recombinant human serum albumin (rHSA) is an important alternative to plasmaderived HSA, but its microbial production is constrained by the secretion burden associated with its large size and 17 intramolecular disulfide bonds.. Here, we developed a systematic secretory-network engineering strategy in the food-safe yeast Kluyveromyces marxianus.. The α-factor leader enabled efficient secretion and correct N-terminal processing of rHSA.

    Isolation:

    Article Title: ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns.
    Article Snippet: .. 2.6 Quantitative real-time PCR (qRT-PCR) Total RNA was isolated using TRIzol Invitrogen (#15596018, Invitrogen) according to the manufacturer’s instruction and was reverse transcribed by using PrimeScript RT reagent Kit (#RR037Q, Takara, Kusatsu, Shiga, Japan). .. Real time PCR was performed using TB Green Premix Ex Taq II (#RR820Q, Takara) on a Real time PCR system (#ABI7900HT, Applied Biosystems, Carlsbad, California).

    Synthesized:

    Article Title: Dahuang Fuzi decoction attenuates renal fibrosis via suppression of the NF-κB pathway.
    Article Snippet: 1 Department of Traditional Chinese Medicine, The Second Affiliated Hospital of Xi’an Jiaotong University, No.157, Xiwu Road, Xincheng District, Xi’an 710004, Shaanxi, China Abstract Background Renal fibrosis is a key pathological process in chronic kidney disease with limited treatment options.. Dahuang Fuzi Decoction (DFT), a classic traditional Chinese medicine formula, shows therapeutic potential, but its anti-fibrotic mechanism remains unclear.. Methods The anti-fibrotic effect of DFT was assessed in a unilateral ureteral obstruction (UUO) rat model via histopathological examination and renal function tests.

    Article Title: Non-coding RNA levels integrating analysis revealed the salt and drought stress response mechanism of miR395e-PvUXS1 pathway in switchgrass.
    Article Snippet: Soil salinization and drought are major constraints on global agricultural production, yet the shared molecular mechanisms by which forage crops respond to these stresses remain incompletedly understood.. Identifying candidate genes with potential value for stress-resilient breeding is therefore an important research objective.. Switchgrass (Panicum virgatum L.) is a perennial forage and bioenergy crop with adaptation to saline, semi-arid, and drought-prone environments.

    Analysis:

    Article Title: Non-coding RNA levels integrating analysis revealed the salt and drought stress response mechanism of miR395e-PvUXS1 pathway in switchgrass.
    Article Snippet: Soil salinization and drought are major constraints on global agricultural production, yet the shared molecular mechanisms by which forage crops respond to these stresses remain incompletedly understood.. Identifying candidate genes with potential value for stress-resilient breeding is therefore an important research objective.. Switchgrass (Panicum virgatum L.) is a perennial forage and bioenergy crop with adaptation to saline, semi-arid, and drought-prone environments.



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